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    Anti-EGFP tag Mouse mAb

    Catalog number :AT0041
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    Overview
    Description
    anti-EGFP-Tag mouse monoclonal antibody
    Reactivity
    Any organism expressing EGFP.
    Tested applications
    WB : 1/1000-1/10000. 
    IHC : 1/200-1/5000.
    ICC/IF : 1/200-1/2000.
    IP : 1/100-1/500.
    Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
    Specificity
    This antibody detects EGFP -tagged proteins exogenously expressed in cells or E. coli. This antibody does not detect RFP-tagged proteins. 
    Please note that the EGFP tags add approximately 27 kDa to the molecular weight of the fusion protein.
    The antibody recognizes the EGFP tag, which is fused to either the amino-terminus or carboxy-terminus of the target protein.
     
    Properties
    Immunogen
    a Enhanced Green Fluorescent Protein (EGFP) recombinant protein.
    Clonality
    7K15
    Isotype
    Mouse IgG1
    Form
    Liquid
    Mouse monoclonal antibody in 10 mM PBS, pH 7.4, 50% glycerol with 1 mg/mL (0.1% w/v) BSA (IgG free, protease free) as a stabilizer and 0.02% (w/v) sodium azide as preservative.
    Storage instruction
    store at -20°C long term. Avoid repeated freeze / thaw cycles.
    Applications
    WB Image

    Western blot - anti-EGFP-Tag mouse mAb (AT0041)
     
    All lanes: 
    Lane: HEK293T cell lysate transfected with EGFP-Tagged protein, anti-EGFP-Tag mouse monoclonal antibody at 1/5000 dilution
     
    Lysates/proteins at 25 µg per lane.
     
    Recommended secondary antibody: Goat anti mouse IgG-HRP (Engibody, Catalog number: AT0098)
    Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)
     

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    All our products are For Research Use Only. Not for diagnostic or therapeutic usages.