Maltose binding protein (MBP) is a useful affinity tag that can increase the expression level and solubility of the MBP tagged protein. It promotes proper folding of the fusion protein, and it can be also used to prevent an insoluble form (inclusion bodies). MBP-tag antibody is suitable for detecting fusion proteins that contain a MBP-tag.
Anti-MBP tag Mouse mAb
Catalog number :AT0030
- Overview
- Description
- Mouse monoclonal antibody to MBP-Tag [7B10] - Epitope Tag Antibody
- Reactivity
- Species independent
- Tested applications
- WB : 1/1000-1/5000.Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
- Product Picture
- Specificity
- This antibody detects recombinant proteins fused to maltose binding protein (MBP).
- Properties
- Immunogen
- Recombinant full-length MBP protein.
- Clonality
- 7B10
- Isotype
- Mouse IgG1
- Form
- Liquid, 100 μg at 1mg/ml
Mouse monoclonal antibody in 10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol with 1 mg/mL (0.1% w/v) BSA (IgG free, protease free) as a stabilizer and 0.02% (w/v) sodium azide as preservative.
- Storage instruction
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C
- Applications
- WB Image
Western blot analysis of MBP-tag fusion protein with anti MBP-tag mouse mAb [7B10] diluted at 1: 2000.
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