Anti-VSV-G tag Mouse mAb

Overview

Description

anti-VSV-G-Tag mouse monoclonal anibody

Reactivity

The antibody recognizes an epitope corresponding to aa 501-511 of vesicular stomatitis virus glycoprotein (VSV-G). This vector is a pseudo lentiviral vector. Peptide sequence: YTDIEMNRLGK

Tested applications

WB    : 1/1000-1/5000.

 

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Properties

Immunogen

Synthetic peptide YTDIEMNRLGK, conjugated to KLH.

Clonality

8K12

Isotype

Mouse IgG1

Form

Liquid
pH: 7.40
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.0268% PBS, 0.164% Sodium phosphate, 1% BSA

Storage instruction

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Applications

WB Image

Western blot - VSV-G tag antibody (AT0029)

All lanes : Anti-VSV-G tag antibody at 1/1000 dilution

Lane 1 : recombinant VSV-G tagged fusion protein at 0.05 µg
Lane 2 : recombinant VSV-G tagged fusion protein at 0.025 µg
Lane 3 : recombinant VSV-G tagged fusion protein at 0.01 µg
Lane 4 : recombinant VSV-G tagged fusion protein at 0.005 µg


Observed band size : 120 kDa