Anti-beta Tubulin Rabbit mAb - Loading Control

Overview

Description

anti-β-Tubulin Rabbit monoclonal antibody

Reactivity

Mouse, Rat, Human

Tested applications

WB: 1/1000-1/10000. 

ICC/IF: 1/200-1/2000.

Optimal dilutions/concentrations should be determined by the end user.

Product Picture

Properties

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta Tubulin.

Clonality

Monoclonal, clone number: 4D6

Isotype

Rabbit IgG

Form

Liquid
Preservative: None
Constituents: 50% Glycerol, PBS, pH 7.4

Storage instruction

Store at -20°C. Avoid freeze / thaw cycle.

Applications

WB Image

All lanes : Anti-beta Tubulin antibody - Loading Control at 1/5000 dilution

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 30µg per lane.

developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 50 kDa
Observed band size : 50 kDa

ICC/IF Image

ICC/IF image of beta Tubulin staining in HeLa cells.
 

The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the anti-beta Tubulin primary antibody (Engibody, Cat no: AT0011, Anti-beta Tubulin Rabbit mAb, 1:500) at 25°C for 0.5 hour. The secondary antibody (green) was Goat Anti-Rabbit IgG(H+L) (iFluor™ 488) (Engibody, Cat no: AT1546, used at 1:500 for 0.5 hour). DAPI was used to stain the cell nuclei (blue).