Anti-GAPDH Rabbit mAb - Loading Control

Product

Anti-GAPDH Rabbit mAb - Loading Control

Catalog number :AT0010

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Overview

Description: anti-GAPDH Rabbit monoclonal antibody

Reactivity: Mouse, Rat, Human

Tested applications: WB: 1/2000-1/10000

Not yet tested in other applications.

Optimal dilutions/concentrations should be determined by the end user.

Product Picture

Properties

Immunogen: Full length native protein from human erythrocytes.

Clonality: Monoclonal, clone number: 3B1

Isotype: Rabbit IgG

Form: Liquid
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Applications

WB Image: All lanes : Anti-GAPDH antibody at 1/5000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : K562 cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : HL60 cell lysate
Lane 8 : SKN-SH cell lysate
Lane 9 : SKBR-3 cell lysate

Predicted band size : 36 kDa