Anti-beta Actin Rabbit mAb - Loading Control

Overview

Description

anti-β-Actin Rabbit monoclonal antibody

Reactivity

Mouse, Rat, Human

Tested applications

WB:1/1000-1/10000

Not yet tested in other applications.

Optimal dilutions/concentrations should be determined by the end user.

 

Product Picture

Properties

Immunogen

Synthetic peptide derived from within residues 1 - 100 of Human beta Actin.

Clonality

Monoclonal, clone number: 9E6

Isotype

Rabbit IgG

Form

Liquid
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4

Storage instruction

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Applications

WB Image

All lanes : Anti-beta Actin antibody - Loading Control (AT0009) at 1:5000
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

Lysates/proteins at 30 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 42 kDa
Observed band size : 42 kDa

WB Figure 1

Western Blot: Beta Actin Rabbit Monoclonal Antibody (AT0009)

Analysis of Beta Actin on different lysates using this antibody at 1:1000 dilution. 

 

Lane 1: HEK293, Lane 2: Hela, Lane 3: PC-12, Lane 4: NIH/3T3