Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
Anti-alpha Tubulin Rabbit mAb - Loading Control
Catalog number :AT0012
- Overview
- Description
- anti-alpha-Tubulin Rabbit monoclonal antibody
- Reactivity
- Mouse, Rat, Human
- Tested applications
- WB: 1/1000-1/10000,
Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
- Properties
- Immunogen
Recombinant fragment, corresponding to a region within amino acids 277-450 of Human alpha Tubulin (Uniprot ID = Q71U36).
- Clonality
- Monoclonal, clone number: 4A7
- Isotype
- Rabbit IgG
- Form
- Liquid
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
- Storage instruction
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles.
- Applications
- WB Image
All lanes : Anti-alpha Tubulin antibody at 1/2000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Mouse brain tissue
Lane 3 : Rat brain tissue
Predicted band size : 54 kDa
Observed band size : 54 kDa
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