Anti-alpha Tubulin Mouse mAb

Product

Anti-alpha Tubulin Mouse mAb - Loading Control

Catalog number :AT0007

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins.

Overview

Description: Mouse monoclonal antibody to α-Tubulin [4F3] - Loading Control

Reactivity: Human, Rat, Mouse

Tested applications: WB : 1/1000-1/10000. Predicted molecular weight: 50 kDa.

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Properties

Immunogen: Recombinant full length protein, corresponding to amino acids 1-452 of Rat alpha Tubulin

Clonality: 4F3

Isotype: Mouse IgG1

Form: Liquid, 100 μg at 1mg/ml

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C

Applications

WB Image: Western blot analysis of 40µg of cell lysates at 1:1000 dilution using Alpha Tubulin antibody

Predicted band size: 50KDa, Observed band size: 50KDa

Lane 1: A431 cells

Lane 2: BT-474 cells

Lane 3: NIH/T3 cells

Lane 4: PC12 cells

Recommended secondary antibody: Goat anti mouse IgG-HRP (Engibody, Catalog number:AT0098)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)