General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
Anti-TATA binding protein(TBP) Rabbit mAb - Nuclear Loading Control
Catalog number :AT0017
- Overview
- Description
- Anti-TATA binding protein TBP Rabbit monoclonal antibody
- Reactivity
- Human, Rat, Mouse
- Tested applications
- WB: 1/500 - 1/3000. Predicted molecular weight: 38 kDa.
- Properties
- Immunogen
Recombinant protein fragment corresponding to a region within amino acids 208 and 339 of Human TATA binding protein TBP (NP_003185).
- Clonality
- Monoclonal, clone number: 5F6
- Isotype
- Rabbit IgG
- Form
- Liquid
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
- Storage instruction
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Applications
- WB Image
Anti-TATA binding protein TBP antibody at 1/500 dilution + 293T whole cell lysate at 30 µg
Predicted band size : 38 kDa
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