Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA protein expression is a well-accepted marker of proliferation.
Anti-PCNA Mouse mAb - Nuclear Loading Control
Catalog number :AT0016
- Overview
- Description
- anti-PCNA(proliferating cell nuclear antigen) mouse monoclonal antibody
- Reactivity
- Human, Mouse, Rat
- Tested applications
- WB(1:4000-1:10000);Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
- Properties
- Immunogen
- Synthetic peptide corresponding to residues near the C terminus of Human PCNA.
- Clonality
- 5G5
- Isotype
- Mouse IgG1
- Form
- Liquid,
Preservative: None
Constituents: PBS
- Storage instruction
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Applications
- WB Image
Western blotAll lanes : Anti-PCNA antibody (AT0016) at 1:5000Lane 1 : HeLa whole cell lysate at 40 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique
Related Products
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