Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Anti-Histone H2A.X Rabbit mAb
Catalog number :AT0015
- Overview
- Description
- anti-Histone H2A.X Rabbit monoclonal antibody
- Reactivity
- Human, Mouse, Rat
- Tested applications
- WB: 1:100-2000;
IHC:1:10-200;
IF:1:10-100;
ChIP, CUT&RUN and CUT&Tag
Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
- Properties
- Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (SGRGKTGGKA RAKAKSRSSR AGLQFPVGRV HRLLRKGHYA ERVGAGAPVY) of Human Histone H2A.X (NP_002096).
- Clonality
- Monoclonal, clone number: 4DB21
- Isotype
- Rabbit IgG
- Form
- 1 mg/ml, Liquid
- Storage instruction
- Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Run BLAST with 
- Applications
- WB Image
Anti-Histone H2A.X antibody at 1:500+ HepG2 cell lysate at 40 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/5000 dilution
Predicted band size : 15 kDa
Observed band size : 15 kDa
Related Products
| Catalog number | AT0005 |
| Catalog number | AT0016 |
| Catalog number | AT0017 |
| Catalog number | AT0018 |
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