Anti-Myc tag Rabbit mAb conjugated Agarose Beads

Overview

Description

Anti-Myc-Tag Rabbit monoclonal antibody conjugated Agarose beads

Reactivity

The antibody detects Myc-Tag proteins overexpressed in cells.

Tested applications

IP. Optimal working dilutions must be determined by end user.

Properties

Immunogen

This antibody is produced by immunizing Rabbits with a synthetic peptide (KLH-coupled) corresponding to residues 410-419 of human c-Myc (EQKLISEEDK).

Clonality

Monoclonal, clone number: 5D12

Isotype

Rabbit IgG

Form

The product is supplied as a 50% slurry in storage buffer (1× PBS, pH 7.4, containing 0.1% NaN3).

Storage instruction

Store at +4°C and do not freeze.

Applications

IP/CoIP Image

HEK 293T cells and Hela were transfected by the same Myc-tagged protein, and 100μl cell lysate (about 100μg total protein) was incubated with 30μl 50% slurry of Anti-Myc antibody Agarose (AT0541) for 3 h at 4°C. After washing, the beads were eluted by 30μl elution buffer twice. After neutralization of the eluant, 6μl 6× SDS loading buffer was added. Then 20μl sample was subjected to the SDS-PAGE. Blot was probed with Anti-Myc-Tag Mouse mAb.

Lane 1: HEK 293T cells.
Lane 2: Hela.