Protein G Magnetic Beads for Antibody Purification

Product

Protein G Magnetic Beads for Antibody Purification

Catalog number :P2712

Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native Protein G has three IgG binding domains and also the sites for albumin and cell-surface binding. Albumin and cell-surface binding domains have been eliminated from recombinant Protein G to reduce nonspecific binding. Protein G has greater affinity than Protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike Protein A, Protein G does not bind to human IgM, IgD and IgA.

Overview

Description: Protein G Magnetic Beads for Antibody Purification

Tested applications: Small-scale Antibody Purification

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Specificity: Protein A and G are popular choices for antibody purification, because they are both stable and target selective. IgG class antibodies from multiple species bind to protein A and/or G, allowing antibody to be captured on protein-bound beads. Protein A and G bind IgG subtypes with varying affinities, determined by species and the properties of the heavy chain. This chart that reports the affinity of protein A and G for various species immunoglobulin subtypes.

Properties

Form: 2ml 25% slurry

Storage instruction: Store at 4℃, DO NOT freeze.

Beads Diameter: average 40 μm

Binding Capacity: more than 10 mg mouse IgG per 1 ml settled beads (e.g. 4 ml 25% slurry)

Ligand: Recombinant protein G produced in E.coli

Matrix: Cross-linked agarose beaded particles containing the superparamagnetic core

Chemical stability: All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.

pH stability: Long term: pH2 - 9;

Short term: pH2 - 11

Storage buffer: 1x PBS containing 0.02% NaN3

Applications

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Highlights: High efficiency – equivalent or higher yield of IP target antigens than magnetic beads from other suppliers

Low non-specific binding – stable, pre-blocked beads provide highly purified product (e.g., antigen eluted in IP with antibody is devoid of contaminating protein from complex cell lysate)

Consistent – magnetic beads eliminate resin loss and provide for more efficient separation than traditional IP methods that use only centrifugation