Protein A Magnetic Beads for Antibody Purification

Product

Protein A Magnetic Beads for Antibody Purification

Catalog number :P2611

Protein A Magnetic Beads are used for purifying antibody from serum, cell culture supernatant or ascites, as well as for IP/Co-IP of antigens from cell or tissue extracts. Protein A can bind to antibodies from many different species, including mouse, human, rabbit, pig, dog, and cat. The protocol for the Protein A beads has been optimized to allow for high recovery and high purity of the isolated antibody or antigen. Antibody or antigen/antibody complex (IP) is first captured on the magnetic beads. The beads are washed and then the target is eluted with low pH elution buffer. IP performance is equivalent to or better than Protein A magnetic beads from other suppliers.

Overview

Description: Protein A Magnetic Beads for Antibody Purification

Tested applications: Antibody Purification

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Specificity: Protein A and G are popular choices for antibody purification, because they are both stable and target selective. IgG class antibodies from multiple species bind to protein A and/or G, allowing antibody to be captured on protein-bound beads. Protein A and G bind IgG subtypes with varying affinities, determined by species and the properties of the heavy chain. This chart that reports the affinity of protein A and G for various species immunoglobulin subtypes.

Properties

Form: 2ml 25% slurry

Storage instruction: 2 - 8°C
Attention: DO NOT freeze

Beads Diameter: average 40 μm

Binding Capacity: more than 10 mg Rabbit IgG per 1 ml settled beads (e.g. 4 ml 25% slurry)

Ligand: Recombinant protein A produced in E.coli

Matrix: Cross-linked agarose beaded particles containing the superparamagnetic core

Chemical stability: All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.

pH stability: Long term: pH2 - 9;

Short term: pH2 - 11

Storage buffer: 1x PBS containing 0.02% NaN3

Applications

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Highlights: Superparamagnetic core with fast response to magnetic field

Low nonspecific binding for assay accuracy

High density of the Protein A ligand for optimal yield of antibodies and Ig fragments

High stability in common buffer systems and detergents