Anti-PCNA Rabbit mAb - Nuclear Loading Control

Product

Anti-PCNA Rabbit mAb - Nuclear Loading Control

Catalog number :AT1577

Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA protein expression is a well-accepted marker of proliferation and PCNA antibody has been used to assess PCNA levels in hundreds of scientific studies.

Overview

Description: Rabbit monoclonal to PCNA

Reactivity: Human,Mouse,Rat
This PCNA antibody recognizes endogenous levels of total PCNA protein.

Tested applications: WB(1:2000-1:10000)

Properties

Immunogen: Recombinant fragment, corresponding to a region within amino acids 1-253 of PCNA (Uniprot: P12004).

Clonality: Monoclonal, clone number: 9C1

Isotype: Rabbit IgG

Storage instruction: Store at -20°C. Aliquot to avoid repeated freezing and thawing.

Applications

Application Image: All lanes : Anti-PCNA antibody (AT1577) at 1/5000 dilution

Lane 1 : 293T whole cell lysate

Lane 2 : NIH 3T3 whole cell lysate

Lane 3 : PC12 whole cell lysate

Lysates/proteins at 30 µg per lane.

Predicted band size : 36 kDa

Anti-PCNA Rabbit mAb - Nuclear Loading Control

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