The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.
Lamin B1 and Lamin B antibodies are extremely useful as nuclear loading controls for use with nuclear extracts. When using Lamin B1 antibodies as nuclear loading controls, be aware that in apoptotic cells Lamin B1 is cleaved. Lamin B1 will also be removed from a nuclear prep if the nuclear membranes are spun out. This antibody was designed to be a nuclear loading control however it has not yet been tested in appropriate lysates.
Anti-Lamin B1 Rabbit mAb - Nuclear Loading Control
Catalog number :AT1585
- Overview
- Description
- anti-Lamin B1 Rabbit monoclonal antibody
- Reactivity
- human,mouse,rat
- Tested applications
- WB:1/1000-1/5000
- Product Picture
- Properties
- Immunogen
- Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Mouse Lamin B1.
- Clonality
- Rabbit monoclonal, clone number: 8A9
- Form
- Liquid
Preservative: 0.02% Sodium AzideConstituents: 1% BSA, PBS, pH 7.4
- Storage instruction
- Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- Database links
- Database link: P20700
- Applications
- WB Image
All lanes : Anti-Lamin B1 antibody - Nuclear Envelope Marker at 1:2000 dilutionLane 1 : Hela whole cell lysateLane 2 : NIH 3T3 whole cell lysateLysates/proteins at 30 µg per lane.SecondaryHRP-Goat anti Rabbit IgG at 1:5000 dilutionPerformed under reducing conditions.Predicted band size : 70 kDaObserved band size : 70 kDa
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