Anti-Lamin A + C rabbit mAb - Nuclear Loading Control

Product

Anti-Lamin A + C rabbit mAb - Nuclear Loading Control

Catalog number :AT0797

LMNA gene conducts alternative splicing to produce Lamin A protein and Lamin C protein.

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization. Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment. The cleavage of lamins results in nuclear dysregulation and cell death.

Database Links
UniProt ID: P02545 LMNA
Entrez-Gene ID: 4000 LMNA

Overview

Reactivity: Mouse, Rat, Human

Tested applications: WB : 1/1000-1/5000.

Predicted molecular weight: 74kDa (Lamin A)/63kDa (Lamin C), 28 kDa(The cleaved smaller fragments).

IHC: 1/50~1/200

ICC/IF: 1/50~1/200

IP: 1/50

FC: 1/50

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody recognizes endogenous levels of 74kDa (Lamin A) and 63kDa (Lamin C). It also reacts with the smaller fragments of lamin A (28 kDa) and lamin C (28 kDa) produced by caspase 6 cleavage during apoptosis. This antibody does not cross-react with lamins B1 and B2.

Properties

Immunogen: a recombinant fragment of human lamin A protein.

Clonality: Monoclonal, clone number: 3G1

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at -20°C, Avoid freeze / thaw cycle. Do not aliquot the antibody.

Host: Rabbit

Applications

WB Image: Western Blotting Image
Predicted band size: 74kDa (Lamin A) and 63kDa (Lamin C)

Anti-Lamin B1 antibody at 1:2000

Western Blot: Lamin A + C Antibody - Wild-type (WT) and Lamin A + C knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was incubated with Lamin A + C antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)

WB Figure 2: Western Blotting Image

Predicted band size: 74kDa (Lamin A)/63kDa (Lamin C)

Anti-Lamin A/C antibody at 1:2000, Cell lysates of Hela

Recommended secondary antibody: Goat anti Rabbit IgG-HRP (Engibody, Catalog number:AT0097)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)

Anti-Lamin A + C rabbit mAb - Nuclear Loading Control

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