Anti-GFP tag Mouse mAb (HRP)

Overview

Description

Mouse monoclonal antibody to GFP[7D10] Conjugated Peroxidase (HRP)

Tested applications

WB : 1/5000-1/10000. 
Optimal dilutions/concentrations should be determined by the end user.

Specificity

The antibody recognizes the GFP, EGFP fused to either the amino or carboxy terminus of targeted proteins in transfected cells. 

There is no need using secondary antibody.

Properties

Immunogen

a Green Fluorescent Protein (GFP) recombinant protein.

Clonality

7D10

Isotype

Mouse IgG2a

Form

10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol with 10 mg/mL (1% w/v) BSA (IgG free, protease free) as a stabilizer and 0.01% (w/v) thimerosal as preservative.

Storage instruction

Store at -20°C, Avoid freeze / thaw cycle. Do not aliquot the antibody.

Applications

WB Image

Western Blot: GFP Antibody-HRP- Analysis of extracts from Hela cells,  transfected with GFP expression vector, using GFP antibody-HRP (AT0055) at 1:5000.
 
Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)