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    mCherry-Alpaca-HRP™ Anti-mCherry tag Alpaca Nanobody(VHH) Conjugated HRP

    Catalog number :AT8322
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    mCherry-Alpaca-HRP™ Anti-mCherry tag Alpaca Nanobody(VHH) Conjugated HRP is designed for detecting mCherry tag fusion proteins specifically. mCherry-Alpaca-HRP™ Anti-mCherry tag Alpaca Nanobody(VHH) Conjugated HRP is recombinant alpaca nanobody conjugated HRP. It detects the mCherry tag selectively, no reactivity with other proteins.

    mCherry is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals). The mCherry protein was derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma.
    Overview
    Description
    mCherry-Alpaca-HRP™ Anti-mCherry tag Alpaca Nanobody(VHH) Conjugated HRP
    Reactivity
    Detect transfected mCherry tag fusion proteins expressed in E. coli, yeast, insect cell, mammalian cell, or in vitro transcription/translation systems. All systems that express mCherry tag fusion protein are suitable.
    Tested applications
    WB:1/2000-1/10000,
    ELISA:1/2000-1/10000
     
    There is no need to use secondary antibody
    Properties
    Immunogen
    mCherry recombinant protein
    Clonality
    Alpaca monoclonal nanobody(VHH,single domain antibody), without Fc fusion
    Form
    Liquid
    Storage instruction
    Store at –20 °C, (Avoid freeze / thaw cycles)
    Conjugation
    Conjugated HRP(Horseradish Peroxidase)
    Host
    Alpaca pacous
    mCherry-Alpaca-HRP™ Anti-mCherry tag Alpaca Nanobody(VHH) Conjugated HRP
    Storage buffer
    In 10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol with 0.02% (w/v) ProClin as preservative.
    Purity
    Recombinant expression in yeast and affinity purified
    Applications
    Highlights
    Benefits Of Alpaca Nanobody
     
    No Fc fragment of ordinary antibodies, so there is no non-specific binding of Fc to the cell surface Fc receptor of immune cells in flow cytometry experiments
     
    Highly specific binding
     
    Low background
     
    High affinity
     
    High stability
     
    Alpaca nanobody is a small molecule with molecular weight of only 13kD (Conventional rabbit or mouse antibody is 150kD), so it can easily enter permeablized cells
     
    Animal-free production, recombinant expression in yeast and affinity purified

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    All our products are For Research Use Only. Not for diagnostic or therapeutic usages.