FLAG-Alpaca-iFluor594™ Anti-Flag tag Alpaca Nanobody (VHH), labelled iFluor™ 594

Overview

Description

FLAG-Alpaca-iFluor594™ Anti-Flag tag Alpaca Nanobody (VHH), labelled  iFluor™ 594

Tested applications

IF/ICC : 1:50-1:1000(dilution)

Flow Cytometry : Particularly suitable for a flow experiment

 

Because there is no Fc fragment of ordinary antibodies, there is no non-specific binding of Fc to the cell surface Fc receptor of immune cells in flow cytometry experiments

 

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Product Picture

Product Picture 1

Figure 1. Alpaca antibodies are smaller than other mammalian antibodies. Mammalian IgG consists a heavy (green) and light (blue) chain, with variable heavy (VH) and variable light (VL) domains that facilitate the memory-like binding properties of antibodies. Camelid antibodies consist only a heavy chain (green) with a single variable heavy (VHH) domain for target recognition. This VHH domain can be expressed alone without the heavy chains, forming a single domain VHH.

 

Product Picture 2

 

Figure 2. Comparison of antibody types. Schematic representation of the comparison between (A) conventional antibodies, (B) alpaca heavy-chain antibodies, (C) and single domain antibodies.

Properties

Immunogen

Synthetic peptide DYKDDDDK conjugated to KLH

Isotype

Alpaca VHH, Single Domain antibody

Form

In 10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol with 4 mg/mL BSA (IgG free, protease free) as a stabilizer and 0.02% ProClin 300 as preservative.

Storage instruction

Store at -20°C, Avoid freeze / thaw cycle. 

Conjugation

iFluor 594
Excitation/Emission maxima wavelengths: 593 nm / 618 nm

Host

Applications

Highlights

Benefits Of Alpaca Nanobody

 

1. No Fc fragment of ordinary antibodies, so there is no non-specific binding of Fc to the cell surface Fc receptor of immune cells in flow cytometry experiments

 

2. iFluor 594 coupled nanobody, one step ICC and Flow Cytometry

 

3. Highly specific binding

 

4. Low background

 

5. High affinity

 

6. High stability

 

7. Alpaca nanobody is a small molecule with molecular weight of only 13kD (Conventional rabbit or mouse antibody is 150kD), so it can easily enter permeablized cells