Anti-ZO-1 Rabbit mAb - Intercellular Tight Junction Marker
Catalog number :AT1791
Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces. Zona occludens proteins ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton. ZO-1 and -2 are required for tight junction formation and function. In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import/export of transcriptional regulators. The ZO-2 gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms. ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms. While both isoforms appear to be widely expressed, abnormal regulation of the ZO-2 gene may be correlated with development of ductal cancer.
- Overview
- Reactivity
- Mouse, Rat, Human
- Tested applications
- Western Blot: 1:1000Immunohistochemistry: 1:500Immunoprecipitation: 1:100
- Specificity
- Recognizes endogenous levels of total ZO-1 protein.
- Properties
- Immunogen
- Produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1558 of human ZO-1 protein.
- Clonality
- Monoclonal, clone number: 5D21
- Isotype
- Rabbit IgG
- Storage instruction
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C
- Applications
- WB Image
Knockout Validated: Tight Junction Protein 1 Antibody - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TJP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:500 of Rabbit Anti-Human ZO1 Polyclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody). Specific band was detected for ZO1 at approximately 260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
- ICC/IF Image
Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody - Staining of human cell line U-2 OS shows localization to cytosol & cell junctions. Antibody staining is shown in green.
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