Anti-V5 tag Mouse mAb (HRP)

Product

Anti-V5 tag Mouse mAb (HRP)

Catalog number :AT0058

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. The V5 tag can be used to detect expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.

Overview

Description: HRP conjugated Mouse monoclonal antibody to V5

Reactivity: V5-Tag mouse mAb (HRP Conjugated) detects transfected V5 fusion proteins expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems.

Tested applications: WB : 1/500-1/1000.

Optimal dilutions/concentrations should be determined by the end user.

Properties

Immunogen: Monoclonal antibody is produced by immunizing animals with a synthetic V5-tag peptide.

Isotype: Mouse IgG1

Form: Liquid

Preservative: 15mM Sodium Azide

Constituents: PBS, pH 7.4

Storage instruction: Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Applications

WB Image: Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected with V5-tag fusion protein (+), using V5-Tag mouse mAb and β-Actin mouse mAb(AT0001)

Anti-V5 tag Mouse mAb (HRP)

Related Products

Reviews