Anti-TSG101 Rabbit mAb - Exosome Marker

Product

Anti-TSG101 Rabbit mAb - Exosome Marker

Catalog number :AT1607

Background:

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.

UniProt ID: Q99816 Human

Overview

Reactivity: Human, Mouse, Rat, Bovine, Pig, Monkey

Tested applications: Western Blotting: 1/1000-1/2000

Immunofluorescence: 1/500

Immunohistochemistry (Paraffin): 1/500

Immunoprecipitation (IP): 1/100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody detects endogenous levels of total TSG101 protein.

Properties

Immunogen: This antibody was developed against Recombinant Protein

Clonality: Monoclonal, clone number: 5D7

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Genetic Strategies Validation. Western Blot: TSG101 Antibody - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TSG101 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

IHC Image: Immunohistochemistry-Paraffin: TSG101 Antibody- Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: TSG101 Antibody - Staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.

Anti-TSG101 Rabbit mAb - Exosome Marker

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