Smart-acRIP™ ac4C RIP Kit

RNA modification was initially regarded merely as a chemical fine-tuning of RNA in nucleic acid structure. With the development of cutting-edge research techniques and the emergence of related antibodies, it has been discovered that RNA modification is widely present at the transcriptome level and exists in a reversible and dynamic regulatory state. It participates in multiple aspects of biological processes, including: cell differentiation, sex determination, tumor occurrence and metastasis, DNA damage repair and other biological functions. 

 

On December 13, 2018, Professor Shalini Oberdoerffer from NIH in the United States published a study in CELL: Acetylation of Cytidine in mRNA Promotes Translation Efficiency. This study first revealed that there are also a large number of ac4C modifications on mRNA and that ac4C modifications affect the stability and translation efficiency of mRNA. Before this, RNA acetylation modification was first discovered in yeast tRNA in 1966 and in rRNA in 1978. 

 

N4-acetylcytidine (ac4C), a conserved chemical modification in both eukaryotes and prokaryotes, was initially believed to mainly exist in tRNA and 18S rRNA. However, recent studies have revealed that a considerable amount of ac4C is also present in mRNA, with its abundance not lower than the m7G cap modification carried by mRNA. N-acetyltransferase 10 (NAT10) is currently the only identified protein that possesses both acetyltransferase and RNA-binding domains, and thus is regarded as the RNA ac4C modification enzyme (the sole known writer protein, while eraser and reader proteins remain unknown).

Overview

Description

Smart-acRIP™ ac4C RIP Kit

Properties

Components

ITEM	NAME	QUANTITY	STORE
STORE AT 4 °C
acRIP-001	acRIP Grade Protein A/G magnetic beads	1 mL	+4 °C	DO NOT FREEZE
acRIP-002	Beads wash buffer	15 mL	+4 °C
acRIP-003	Beads-antibody binding buffer	5 mL	+4 °C
acRIP-004	10× RNA Fragmentation Reagent	150 µL	+4 °C
acRIP-005	10× Stop Buffer	150 µL	+4 °C
acRIP-031	antibody-acRNA binding buffer	15 mL	+4 °C
acRIP-007	acRIP Wash Buffer 1	30 mL	+4 °C
acRIP-008	acRIP Wash Buffer 2	30 mL	+4 °C
acRIP-009	acRIP Elution Buffer	4 mL	+4 °C
GIFT	RNA Extraction R	15 mL	+4 °C
GIFT	RNA Extraction Buffer	15 mL	+4 °C
STORE AT -20 °C
acRIP-019	RNase Inhibitor	50 µL	-20 °C
acRIP-020	Proteinase K	100 µL	-20 °C
acRIP-021	acRIP grade Normal Mouse IgG	100 µL	-20 °C
acRIP-022	acRIP grade Normal Rabbit IgG	100 µL	-20 °C
acRIP-023	RNA  Sinking agent	100 µL	-20 °C
acRIP-006	RNA precipitation solution	1.5 mL	-20 °C
acRIP-024	ac4C RNA RT random primers	20 µL	-20 °C
acRIP-025	ac4C RNA RT mastermix	100 µL	-20 °C
acRIP-026	RIP grade ac4C antibody	24 µL	-20 °C	GIFT

 
 

Applications

Application Image

Highlights

1. This kit is suitable for the analysis of ac4C acetylation of total RNA, mRNA, lncRNA, and circRNA from cells, animal tissues, plant tissues, blood, and embedded tissues.

 

2. This kit is suitable for analyzing cytoplasmic RNA ac4C acetylation and nuclear RNA ac4C acetylation.

 

3. This kit contains ac4C antibody and negative control lgG.

 

4. This kit contains the random primers required for downstream acRIP reverse transcription and the acRIP specific reverse transcriptase mastermix.

 

5. This kit is suitable for downstream RT qPCR and ac4C acetylation RNA library sequencing.

Work Flow Image