RFP-Alpaca-HRP™ Anti-RFP tag Alpaca Nanobody(VHH) Conjugated HRP

Overview

Description

RFP-Alpaca-HRP™ Anti-RFP tag Alpaca Nanobody(VHH) Conjugated HRP

Reactivity

Detect transfected RFP tag fusion proteins expressed in E. coli, yeast, insect cell, mammalian cell, or in vitro transcription/translation systems. All systems that express RFP tag fusion protein are suitable.

Tested applications

WB:1/2000-1/10000,

ELISA:1/2000-1/10000

 

There is no need to use secondary antibody

Properties

Properties

Immunogen

Red Fluorescent Protein (RFP) recombinant protein

Clonality

Alpaca monoclonal nanobody(VHH,single domain antibody), without Fc fusion

Form

Liquid

Storage instruction

Store at –20 °C, (Avoid freeze / thaw cycles)

Conjugation

Conjugated HRP(Horseradish Peroxidase)

Host

Alpaca pacous

Storage buffer

In 10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol with 0.02% (w/v) ProClin as preservative.

Purity

Recombinant expression in yeast and affinity purified

Applications

Highlights

Benefits Of Alpaca Nanobody

 

No Fc fragment of ordinary antibodies, so there is no non-specific binding of Fc to the cell surface Fc receptor of immune cells in flow cytometry experiments

 

Highly specific binding

 

Low background

 

High affinity

 

High stability

 

Alpaca nanobody is a small molecule with molecular weight of only 13kD (Conventional rabbit or mouse antibody is 150kD), so it can easily enter permeablized cells

 

Animal-free production, recombinant expression in yeast and affinity purified