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    Myc-Alpaca-Biotin™ Anti-Myc tag Alpaca Nanobody(VHH) Conjugated Biotin

    Catalog number :AT8313
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    Myc-Alpaca-Biotin Anti-Myc tag Alpaca Nanobody(VHH) Conjugated Biotin is designed for detecting Myc tag fusion proteins specifically. Myc-Alpaca-Biotin Anti-Myc tag Alpaca Nanobody(VHH) Conjugated Biotin is recombinant alpaca nanobody conjugated biotin. It detects the Myc tag selectively, no reactivity with other proteins.
    Overview
    Description
    Myc-Alpaca-Biotin™ Anti-Myc tag Alpaca Nanobody(VHH) Conjugated Biotin 
    Reactivity
    Detect transfected Myc tag fusion proteins expressed in E. coli, yeast, insect cell, mammalian cell, or in vitro transcription/translation systems. All systems that express Myc tag fusion protein are suitable.
    Tested applications
    It can be coupled with streptavidin, or streptavidin-HRP,  or streptavidin-fluoresent dye, or streptavidin-beads.
     
    Applications for which the Streptavidin-biotin interaction is used include:
     
    Enzyme linked immunosorbent assay (ELISA)
    Immunohistochemistry (IHC)
    Western, Northern and Southern blotting
    Immunoprecipitation
    Cell surface labeling
    Affinity purification
    Fluorescence-activated cell sorting (FACS)
    Electrophoretic mobility shift assays (EMSA)
     
    Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
    Properties
    Immunogen
    a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL) conjugated to KLH
    Clonality
    Alpaca monoclonal nanobody(VHH,single domain antibody)
    Form
    Liquid
    Storage instruction
    Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C
    Conjugation
    Conjugated biotin
    Host
    Alpaca pacous
    Anti-Myc tag Alpaca Nanobody(VHH) Conjugated Biotin
    Binding Capacity
    Dissociation constant KD of 0.1nM
    Storage buffer
    In 10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol with 0.02% (w/v) ProClin as preservative.
    Purity
    Recombinant expression in yeast and affinity purified
    Applications
    Highlights
    Benefits Of Alpaca nanobody
     
    No Fc fragment of ordinary antibodies, so there is no non-specific binding of Fc to the cell surface Fc receptor of immune cells in flow cytometry experiments
    Highly specific binding
    Low background
    High affinity
    High stability
    Alpaca nanobody is a small molecule with molecular weight of only 13kD (Conventional rabbit or mouse antibody is 150kD), so it can easily enter permeablized cells
    Animal-free production, recombinant expression in yeast and affinity purified

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    All our products are For Research Use Only. Not for diagnostic or therapeutic usages.