Anti-LAMP1 Rabbit mAb - Lysosome Marker

Product

Anti-LAMP1 Rabbit mAb - Lysosome Marker

Catalog number :AT0807

Background:

Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins. Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis. The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes. LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking.

Overview

Reactivity: Mouse, Rat, Human, Monkey, Pig, Zebrafish

Tested applications: Western Blotting: 1/1000-1/5000

Immunofluorescence: 1/500

Immunohistochemistry (Paraffin): 1/500

Immunoprecipitation(IP) : 1/100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody recognizes endogenous levels of total LAMP1 protein.

Properties

Immunogen: a recombinant protein fragment of human LAMP1 protein.

Clonality: Monoclonal, clone number: 5RE7

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: LAMP-1/CD107a Antibody - Analysis of LAMP-1 in EL4 cell lysate with LAMP-1 antibody at (A) 1 and (B) 2 ug/mL.

IHC Image: Immunohistochemistry: LAMP-1/CD107a Antibody - Staining of rat colon tissue with antibody at 2.5 ug/ml.

ICC/IF Image: Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody - Staining of rat colon tissue with antibody at 20 ug/ml.

Anti-LAMP1 Rabbit mAb - Lysosome Marker

Related Products

Reviews