Anti-HA tag Rabbit mAb

Product

Anti-HA tag Rabbit mAb

Catalog number :AT0046

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Overview

Description: anti-HA-Tag Rabbit mAb

Tested applications: WB : 1/1000-1/5000.

IHC : 1/200-1/500.

ICC/IF : 1/100-1/500.

IP : 1/50-1/200.

Optimal dilutions/concentrations should be determined by the end user.

Product Picture

Properties

Immunogen: YPYDVPDYA(influenza hemagglutinin-HA-epitope) conjugated to KLH.

Clonality: Monoclonal, clone number: 7C10

Isotype: Rabbit IgG

Form: Liquid, 1 mg/ml

Storage instruction: Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Applications

WB Image: All lanes : Anti-HA tag antibody - (AT0046) at 1/1000 dilution

Lane 1 : 15ug untransfected wcl lysate

Lane 2 : 293FT cells transfected with 15kDa HA tagged Vpr (an HIV1 accessory protein)

Secondary

HRP conjugated Goat anti-Rabbit

developed using the ECL technique

Performed under reducing conditions.

Anti-HA tag Rabbit mAb

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