Anti-GST tag Mouse mAb (HRP)

Product

Anti-GST tag Mouse mAb (HRP)

Catalog number :AT0053

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.

Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade.

Overview

Description: HRP conjugated Mouse monoclonal antibody to GST

Reactivity: GST mouse mAb (HRP Conjugated) detects transfected GST fusion proteins expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems.

Tested applications: WB : 1/500-1/1000.

Optimal dilutions/concentrations should be determined by the end user.

Properties

Immunogen: GST fusion protein

Isotype: Mouse IgG1

Form: Liquid

Preservative: 15mM Sodium Azide

Constituents: PBS, pH 7.4

Storage instruction: Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Applications

WB Image: Western blot analysis of extracts from COS-7 cells, transfected with a construct overexpressing GST-tagged protein, using GST mouse mAb (HRP Conjugated) at 1:1000 dilution.

Anti-GST tag Mouse mAb (HRP)

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