Anti-GFP tag Rabbit mAb

Product

Anti-GFP tag Rabbit mAb

Catalog number :AT1598

Green fluorescent protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.

Overview

Tested applications: WB : 1/1000-1/5000.

IHC : 1/200-1/500.

ICC/IF : 1/100-1/500.

Co-IP : 1/50-1/200.
ChIP:10ul for per reaction

Optimal dilutions/concentrations should be determined by the end user.

Product Picture

Specificity: This antibody detects GFP, EGFP -tagged proteins exogenously expressed in cells or E. coli. This antibody does not detect RFP-tagged proteins.

Please note that the GFP, EGFP tags add approximately 27 kDa to the molecular weight of the fusion protein.

The antibody recognizes the GFP, EGFP tag, which is fused to either the amino-terminus or carboxy-terminus of the target protein.

Properties

Immunogen: a Green Fluorescent Protein (GFP) recombinant protein.

Clonality: Monoclonal, clone number: 7D5

Isotype: Rabbit IgG

Form: Liquid, 100 μg at 1mg/ml

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C

Applications

WB Image: Western Blot: GFP Antibody - Analysis of extracts from 293A cells, unstransfected (lane 1) and transfected (lane 2) with GFP expression vector, using GFP antibody at 1:1000.

Anti-GFP tag Rabbit mAb

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