Anti-EEA1 Rabbit mAb - Early Endosome Marker

Product

Anti-EEA1 Rabbit mAb - Early Endosome Marker

Catalog number :AT0796

Background:

EEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking. The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes. The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and calcium/calmodulin. Once on the membrane, EEA1 interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion.

UniProt ID: Q15075

Overview

Reactivity: Human, Mouse, Rat, Bovine, Pig, Monkey

Tested applications: Western Blotting: 1/1000-1/2000

Immunofluorescence: 1/500

Immunohistochemistry (Paraffin): 1/500

Immunoprecipitation(IP) : 1/100

Optimal dilutions/concentrations should be determined by the end user.

Specificity: This antibody detects endogenous levels of total EEA1 protein.

Properties

Immunogen: a synthetic peptide corresponding to residues surrounding Ser70 of human EEA1 protein.

Clonality: Monoclonal, clone number: 5CD7

Isotype: Rabbit IgG

Form: Liquid, 100 μl,1mg/ml, PBS (pH 7.2) and 40% Glycerol，0.02% Sodium Azide

Storage instruction: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Host: Rabbit

Applications

WB Image: Western Blot: EEA1 Antibody - A: Mouse brain 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (was used to detect the primary antibody.

IHC Image: Immunohistochemistry-Paraffin: EEA1 Antibody - Mouse hind brain.

Anti-EEA1 Rabbit mAb - Early Endosome Marker

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