Anti-DYKDDDDK (FLAG®) tag Rabbit mAb

Overview

Description

Rabbit monoclonal antibody to Flag tag - (Binds to same epitope as Sigma's Anti-FLAG M2 Antibody)

Reactivity

All species

Tested applications

WB : 1/1000-1/5000. 

IHC : 1/200-1/500.

ICC/IF : 1/100-1/500.

IP : 1/50-1/200.

Optimal dilutions/concentrations should be determined by the end user.

Product Picture

Properties

Immunogen

Synthetic peptide, DYKDDDDK conjugated to KLH

Clonality

Monoclonal, clone number: 7D1

Isotype

Rabbit IgG

Form

Liquid, 100 ul, at 1mg/ml with PBS, pH 7.4, containing 0.02% sodium azide

Storage instruction

The antibody can be stored for 2-3 weeks at 2-8°C. For long term, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications

WB Image

Lane : 3T3 cells transfected with DYKDDDDK-tagged protein vector at 1:1000.

WB Figure 2

Antibody detects both C-terminal linked and N-terminal linked FLAG tagged recombinant proteins at 1:5000.

 

Recommended secondary antibody: Goat anti Rabbit IgG-HRP (Engibody, Catalog number:AT0097)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)

ICC/IF Image

DYKDDDDK Epitope Tag Antibody - Samples: Human microvascular endothelial cells expressing FLAG tagged beta-catenin following transient transfection. 

 

Anti-DYKDDDDK Epitope Tag Antibody (Cat no: AT0502) used at 1:500. Detection: FITC labeled goat anti-rabbit IgG (H+L).