Anti-DYKDDDDK(FLAG) tag Alpaca-nano™ mouse monoclonal antibody

Overview

Description

Anti-DYKDDDDK tag Alpaca-nano™ mouse monoclonal antibody (Fab from Alpaca)

Reactivity

Detect transfected DYKDDDDK tag fusion proteins expressed in E. coli, yeast, insect cell, mammalian cell, or in vitro transcription/translation systems.

Tested applications

WB    : 1/2000-1/10000.

ELISA    : 1/5000-1/10000.

ICC/IF    : 1/200-1/1000.

IP/CoIP    : 1/100-1/500.

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Properties

Immunogen

Synthetic peptide: DYKDDDDK conjugated to KLH.

Clonality

Monoclonal, clone number: 3A6

Isotype

Fab of alpaca IgG1 fused to mouse IgG2b Fc   

Form

Liquid

Storage instruction

 Store at –20 °C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C

Conjugation

Unconjugated

Host

Alpaca pacous

Binding Capacity

Dissociation constant KD of 0.1nM

Storage buffer

In 10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol and 0.02% (w/v) proclin300 as preservative.

Purity

Recombinant Expression and Affinity purified

Applications

Highlights

High lot-to-lot consistency

 

Increased sensitivity and higher affinity

 

Animal-free production